Kev Siv Ntau Ntawm Nqus Plua Plav Cov Ntshav Cov Ntshav

Liab cap raj: raj nqus plua plav

Txoj kev ntsuam xyuas kab mob feem ntau siv rau kev kuaj ntshav tshuaj biochemical thiab kev sim tshuaj thaum khaws cov ntshav. Sab hauv phab ntsa yog tusyees coated nrog silicone roj los tiv thaiv lub phab ntsa ntawm dai. Nws siv txoj ntsiab cai ntawm kev tso ntshav ntshav kom ua tau cov ntshav coagulate. Tom qab cov ntshav dej tau sib cais lawm, nws tuaj yeem yog centrifuged rau kev sim.

Txiv kab ntxwv cap raj: Coagulation leeg

Nws feem ntau yog siv rau kev sim tshuaj biochemical thaum muaj xwm txheej ceev. Vim tias qhov sib ntxiv ntawm cov coagulant uas tuaj yeem ua kom cov tshuaj fibrin, soluble fibrin tuaj yeem hloov mus ua insoluble fibrin polymer, thiab tom qab ntawd cov fibrin ruaj khov tuaj yeem tsim. Feem ntau, cia nws sawv ntsug li 5 feeb tom qab kuaj ntshav Cov ntshav coagulates thiab tuaj yeem kuaj los ntawm centrifugation.

Qhov pom daj: inert sib cais gel accelerating raj

Nws tau qhuas nws txoj kev ruaj khov. Nws yog ib qho inert sib cais gel uas ua raws li kev cais tawm ntawm lub hauv paus ntawm txoj hlab raj. Tom qab centrifugation, qhov sib cais gel no tuaj yeem cais cov kua sib xyaw ua kua (dej ntshiab) thiab cov khoom sib xyaw (cov roj ntsha) hauv cov ntshav thiab sib sau tag nrho hauv nruab nrab ntawm lub raj ntsuas kab los ua qhov thaiv, thiab nyob ruaj khov tsis pub dhau 48 teev. Nws yuav tsum nco ntsoov tias cov ntshav yuav tsum rov qab thiab sib xyaw ua ke tom qab cov ntshav tau sib sau, thiab sab laug sawv ntsug rau 30 feeb thiab centrifuged. Vim tias nws tus nqi siab, nws yog feem ntau siv rau kev kuaj pom cov thyroid muaj nuj nqi, cov cim qog, PCR thiab qib theem.

Lub taub hau ntsuab: tub rog heparin anticoagulant raj

Peb feem ntau siv cov kab mob heparin-ntxiv anticoagulant thaum peb kuaj kab rau cov menyuam yaus, vim tias heparin tuaj yeem tiv thaiv kev tsim ntawm thrombin thiab kev ua ke ntawm platelets. Txawm li cas los xij, nws yuav tsum raug sau tseg tias lub raj tshuaj sodium heparin tsis tuaj yeem siv thaum kuaj pom sodium ions hauv qhov hnoos qeev; lub raj no kuj tseem tsis tuaj yeem siv rau kev tso ntshav dawb thiab sib cais, vim hais tias heparin tuaj yeem ua rau cov qe ntshav dawb sib xyaw ua ke.

Ntshav cap raj: EDTA anticoagulation tube

Qhov ntsuas kab mob ntshav zoo nkauj no yog lub luag haujlwm ntawm cov ntshav hematology system kuaj, vim hais tias lub ethylenediaminetetraacetic acid (EDTA) hauv nws tuaj yeem zoo chelate calcium ions hauv cov ntshav ntsuas, tshem tawm cov calcium los ntawm qhov chaw tiv thaiv, thaiv thiab nres cov txheej txheem endogenous lossis exogenous coagulation. txwv tsis pub cov kab mob tawg, tab sis nws tuaj yeem ua rau cov lymphocytes tshwm sim zoo li lub paj tawg, thiab tseem tuaj yeem ua rau EDTA-dependent kev sib xyaw ntawm cov platelets. Yog li, nws tsis tuaj yeem siv rau kev sim kev coagulation thiab kuaj ntshav ua haujlwm. Feem ntau, peb rov qab sib xyaw thiab muab cov ntshav tam sim ntawd tom qab cov ntshav tau sib sau, thiab cov qauv xav tau sib xyaw ua ntej qhov ntsuas, tsis muaj centrifugation.

Lub raj xiav xiav: coagulation test tube

Vim tias EDTA cov leeg tsis tuaj yeem siv rau kev sim coagulation, dab tsi siv rau kev sim tshuaj coagulation? Nov yog qhov ntsuas xiav xiav nrog sodium citrate uas kuv xav ua kom paub. Sodium citrate ua raws li anticoagulant los ntawm chelating nrog calcium ions hauv cov ntshav ntsuas. Txij li thaum qhov sib piv ntawm anticoagulant rau cov ntshav yuav tsum yog 1: 9, nws yog qhov yuav tsum tau ua kom ntseeg tau txaus tus nqi ntawm 2 mL kom ntseeg tau tias muaj kev ntseeg tau ntawm cov ntawv sib tw. Tam sim ntawd tom qab kuaj ntshav, invert thiab sib tov zoo kom tsis txhob coagulation.

Dub cap raj: erythrocyte sedimentation tus nqi kuaj raj

Zoo ib yam li kev kuaj mob coagulation, qhov ntsuas erythrocyte sedimentation tus nqi ntsuas cov raj yuav tsum siv 3.2% sodium citrate tube, thiab 2 mL ntawm cov ntshav yuav tsum tau sau los xyuas kom meej tias qhov sib piv ntawm anticoagulant rau ntshav yog 1: 4, vim tias yog qhov sib piv ntawm anticoagulant siab dhau heev lawm, nws yuav Nws yuav ua rau kom ntshav thiab sai sai erythrocyte sedimentation tus nqi. Tom qab khaws ntshav, nws yuav tsum tau rov qab thiab sib xyaw tam sim ntawd kom tsis txhob txhaws.

Grey cap raj: ntshav qabzib lub raj

Lwm txoj kev ntsuas lub raj yog qhov kev sim tshuaj no siv los soj ntsuam cov ntshav qab zib. Nws muaj cov tshuaj anticoagulant potassium oxalate tsis muaj zog lossis sodium fluoride, uas ua lub luag haujlwm zoo hauv kev tiv thaiv ua kom tsis txhob muaj ntshav qab zib. Nws yog ib qho tshuaj zoo heev rau ntshav qab zib thiab yuav tsum tau siv tshuaj maj mam. Invert thiab sib tov zoo. Nws yuav tsum raug sau tseg tias nws tsis tuaj yeem siv rau kev txiav txim siab ntawm urea los ntawm cov txheej txheem tso zis, tsis tuaj yeem siv rau kev kuaj pom ntawm alkaline phosphatase thiab amylase.